| FOL1 | FOL2 | FOL3 | ABZ1 | LIP1 | DFR1 | MIS1 | ADE3 | SHM1 | SHM2 | MET7 | |
| FOL1 | 0.166 | 0.212 | 0.212 | 0.254 | 0.212 | 0.234 | 0.234 | 0.325 | 0.325 | 0.212 | |
| FOL2 | 0.154 | 0.154 | 0.113 | 0.154 | 0.406 | 0.406 | 0.154 | 0.154 | 0.154 | ||
| FOL3 | 0.512 | 0.154 | 0.199 | 0.388 | 0.388 | 0.199 | 0.199 | 0.728 | |||
| ABZ1 | 0.154 | 0.199 | 0.388 | 0.388 | 0.199 | 0.199 | 0.512 | ||||
| LIP1 | 0.154 | 0.158 | 0.158 | 0.260 | 0.260 | 0.154 | |||||
| DFR1 | 0.419 | 0.419 | 0.199 | 0.199 | 0.199 | ||||||
| MIS1 | 1 | 0.204 | 0.204 | 0.388 | |||||||
| ADE3 | 0.204 | 0.204 | 0.388 | ||||||||
| SHM1 | 1 | 0.199 | |||||||||
| SHM2 | 0.199 | ||||||||||
| MET7 |
| Threshold | Initial | 1.000 | 0.720 | 0.510 | 0.410 | 0.400 | 0.380 | 0.320 | 0.260 | 0.230 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clustering Result |
|
|
|
|
|
|
|
|
|
|
| FOL1 | FOL2 | FOL3 | ABZ1 | LIP1 | DFR1 | MIS1 | ADE3 | SHM1 | SHM2 | MET7 | |
| FOL1 | 0.281 | 0.281 | 0.281 | 1.104 | 0.281 | 0.281 | 0.281 | 1.104 | 1.104 | 0.281 | |
| FOL2 | 0.281 | 0.281 | 0.281 | 0.281 | 3.409 | 3.409 | 0.281 | 0.281 | 0.281 | ||
| FOL3 | 4.328 | 0.281 | 0.281 | 2.305 | 2.305 | 0.281 | 0.281 | 4.929 | |||
| ABZ1 | 0.281 | 0.281 | 2.305 | 2.305 | 0.281 | 0.281 | 4.328 | ||||
| LIP1 | 0.281 | 0.281 | 0.281 | 1.378 | 1.378 | 0.281 | |||||
| DFR1 | 3.409 | 3.409 | 0.281 | 0.281 | 0.281 | ||||||
| MIS1 | 6.953 | 0.281 | 0.281 | 2.305 | |||||||
| ADE3 | 0.281 | 0.281 | 2.305 | ||||||||
| SHM1 | 4.977 | 0.281 | |||||||||
| SHM2 | 0.281 | ||||||||||
| MET7 |
| Threshold | Initial | 6.954 | 4.954 | 4.854 | 4.254 | 3.354 | 2.254 | 1.354 | 1.054 | 0.254 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clustering Result |
|
|
|
|
|
|
|
|
|
|
Genes ADE3 and MIS1 are both annotated by three molecular function terms: GO:0004329: formate-tetrahydrofolate ligase activity, GO:0004477: methenyltetrahydrofolate cyclohydrolase activity, and GO:0004488: methylenetetrahydrofolate dehydrogenase (NADP+) activity. Genes SHM1 and SHM2 are annotated by the same molecular function term GO:0004372: glycine hydroxymethyltransferase activity.
Therefore, ADE3 and MIS1 have the same molecular functions. SHM1 and SHM2 have the same molecular function.
The similarity values and clustering results obtained by G-SESAME are consistent with these observations.
However,based on Resnik's method, the similarity between ADE3 and MIS1 is greater than the similarity between SHM1 and SHM2. This is inconsistent with the human perception.
G-SESAME is better.